English translation for "ammonium sulfate fractionation"
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- 硫酸铵分级分离
Related Translations:
- Example Sentences:
| 1. | 30 % ~ 60 % ammonium sulfate fractionation of the crude extract was carried out at 4 ? . the optimum temperature and ph for it were 37 ? and 6 . 5
结果发现，该酶作用的最适ph为6 . 5 ，最适温度为37 ；在ph5 8之间稳定，其热稳定性不高。 | | 2. | The purified badh , which became a single band in sds - page , has been obtained by a combination of ammonium sulfate fractionation , ion exchange chromatography and gel filtration chromatography
辽宁碱蓬甜菜碱醛脱氢酶分子量为129kd ，单个亚基的分子量为64 . 5kd ，表明其为同型二聚体。 | | 3. | The enzyme was purified from cell extract by ammonium sulfate fractionation ( 30 % - 80 % saturation ) and consecutive column chromatography using deae - cellulose and sephadex g - 100 . the sod activity for purified enzyme could reach 4966 iu / mg proteins , and the molecular weight of the sod was about 20 kda which determined by sds - page electrophoresis . when the non - denaturing polyacrylamide gel stained with substrate for sod in the present of 0 . 2mmol / l kcn or 0 . 5 mmol / l h2o2 , the active band was still showed at the same position , which indicated the sod could not be inhibited by the treatments with kcn or h2o2 and it was the mn sod
通过sds一聚丙烯酸胺凝胶电泳可知该sod酶的分子量约为20kda .在非变性聚丙烯酞胺凝胶( pagb )电泳后，在凝胶son显色反应液中加入0 . 2耐01 / lkcn或0 . 5inmol / lh202 ，凝胶sod显色反应后在原来的sod酶部位仍然含有sod活性带，这表明利用kcn和h20 :处理并不能抑制500的活性，该sod属于mn一sod 。 |
- Similar Words：
- "ammonium stannic chloride" English translation, "ammonium stearate" English translation, "ammonium succinate" English translation, "ammonium sulfamate" English translation, "ammonium sulfate" English translation, "ammonium sulfate nitrate" English translation, "ammonium sulfate precipitation" English translation, "ammonium sulfate precipitation method" English translation, "ammonium sulfate precipitation procedure" English translation, "ammonium sulfate test" English translation
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